periodic acid schiff reagent Search Results


90
Fisher Scientific periodic acid–schiff
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
Periodic Acid–Schiff, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA periodic acid and schiff’s reagent for microscopy
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
Periodic Acid And Schiff’s Reagent For Microscopy, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA periodic acid-schiff (pas)
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
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Beijing Solarbio Science periodic acid schiff/alcian blue staining
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
Periodic Acid Schiff/Alcian Blue Staining, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ScyTek Inc periodic acid-schiff
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
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ScyTek Inc periodic acid-schiff stain kit scytek laboratories
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
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Nanjing Jiancheng Bioengineering Research Institute Co Ltd periodic acid–schiff (pas) staining kit
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
Periodic Acid–Schiff (Pas) Staining Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Imeb Inc periodic acid-schiff (pas)
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
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Imeb Inc periodic acid-schiff solution
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
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Beyotime periodic acid-schiff staining
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
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Baso Diagnostics Inc periodic acid-schiff staining
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
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Beyotime periodic acid-schiff (pas) staining kit
( A ) Representative images <t>of</t> <t>immunohistochemistry</t> for p57 kip2 , followed by periodic <t>acid–Schiff</t> post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
Periodic Acid Schiff (Pas) Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Representative images of immunohistochemistry for p57 kip2 , followed by periodic acid–Schiff post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.

Journal: bioRxiv

Article Title: Nicotinamide riboside activates renal metabolism and protects the kidney in a model of Alport syndrome

doi: 10.1101/2024.02.26.580911

Figure Lengend Snippet: ( A ) Representative images of immunohistochemistry for p57 kip2 , followed by periodic acid–Schiff post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.

Article Snippet: Immunohistochemistry for p57 kip2 (Cat. No. ab75975, Abcam) followed by periodic acid–Schiff (Cat. No. 22-110-645, Fisher Scientific, Hampton, NH) post-staining without hematoxylin counterstaining was performed and analyzed as previously described ( ).

Techniques: Immunohistochemistry, Staining, Immunostaining, Western Blot, Marker