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Image Search Results
Journal: bioRxiv
Article Title: Nicotinamide riboside activates renal metabolism and protects the kidney in a model of Alport syndrome
doi: 10.1101/2024.02.26.580911
Figure Lengend Snippet: ( A ) Representative images of immunohistochemistry for p57 kip2 , followed by periodic acid–Schiff post-staining without hematoxylin counterstaining. Podocyte nuclei are stained brown, and all other glomerular structures are stained pink. ( B ) Quantification of p57 kip2 immunostaining with PodoCount, a validated algorithm to analyze p57 kip2 -stained whole slide images. Podocyte volumetric density was reduced in Alport mice and restored by NR treatment in both sexes. ( C,D ) Immunoblots for KIM-1, a tubular injury marker, in male ( C ) and female ( D ) kidney homogenate. Ponceau S, a nonspecific protein stain, was used as a loading control. ( E,F ) Quantification of immunoblots ( C,D ) shows that KIM-1 is increased in Alport mice and reduced by NR treatment in male ( E ) and female ( F ) mice. Scale bars represent 100 µm. Significance was determined by one-way ANOVA with the Holm–Šídák correction for multiple comparisons. Data are expressed as the means ± SEM. Each datum represents one glomeruli ( B ) or one mouse ( E-F ). * P < 0.05, ** P < 0.01, **** P < 0.0001. Kidney injury molecule-1, KIM-1; NR, nicotinamide riboside; PSR, picrosirius red; Veh, vehicle.
Article Snippet: Immunohistochemistry for p57 kip2 (Cat. No. ab75975, Abcam) followed by
Techniques: Immunohistochemistry, Staining, Immunostaining, Western Blot, Marker